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Quantitative Proteomics of Yeast Post-Golgi Vesicles Reveals a Discriminating Role for Sro7p in Protein Secretion

  1. Annabelle Forsmark1,
  2. Guendalina Rossi2,
  3. Ingrid Wadskog3,
  4. Patrick Brennwald2,
  5. Jonas Warringer1,4,
  6. Lennart Adler1,*

Article first published online: 8 APR 2011

DOI: 10.1111/j.1600-0854.2011.01186.x

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Traffic

Traffic

Volume 12, Issue 6, pages 740–753, June 2011

Additional Information

How to Cite

Forsmark, A., Rossi, G., Wadskog, I., Brennwald, P., Warringer, J. and Adler, L. (2011), Quantitative Proteomics of Yeast Post-Golgi Vesicles Reveals a Discriminating Role for Sro7p in Protein Secretion. Traffic, 12: 740–753. doi: 10.1111/j.1600-0854.2011.01186.x

Author Information

  1. 1

    Department of Cell and Molecular Biology, Microbiology, University of Gothenburg, Box 462, SE-405 30 Gothenburg, Sweden

  2. 2

    Department of Cell and Developmental Biology, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA

  3. 3

    School of Engineering, Jönköping University, Box 1026, SE-551 11 Jönköping, Sweden

  4. 4

    Centre for Integrative Genetics (CIGENE), Norwegian University of Life Sciences (UMB), PO Box 5003, 1432 Ås, Norway

*Lennart Adler, lennart.adler@cmb.gu.se

Publication History

  1. Issue published online: 9 MAY 2011
  2. Article first published online: 8 APR 2011
  3. Received 22 April 2010, revised and accepted for publication 1 March 2011, published online 8 April 2011

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Keywords:

  • exocytosis;
  • Golgi;
  • membrane trafficking;
  • proteomics;
  • vesicles;
  • iTRAQ

We here report the first comparative proteomics of purified yeast post-Golgi vesicles (PGVs). Vesicle samples isolated from PGV-accumulating sec6-4 mutants were treated with isobaric tags (iTRAQ) for subsequent quantitative tandem mass spectrometric analysis of protein content. After background subtraction, a total of 66 vesicle-associated proteins were identified, including known or assumed vesicle residents as well as a fraction not previously known to be PGV associated. Vesicles isolated from cells lacking the polarity protein Sro7p contained essentially the same catalogue of proteins but showed a reduced content of a subset of cargo proteins, in agreement with a previously shown selective role for Sro7p in cargo sorting.

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