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Supporting Information

Additional Supporting Information may be found in the online version of this article:

Figure S1: Western blot showing the distribution of Snc1/2p and Sso1/2p in sorbitol vesicle gradient fractions from the sro7Δ strain. Samples were prepared and treated as in Figure 1 and Materials and Methods. Proteins were resolved by SDS–PAGE and analyzed by Snc1/2p and Sso1/2p immunoblots.

Figure S2: Breakdown by known localization of the 91 proteins identified in Table 2. Annotations are from the SGD yeast GO slim component catalogue (http://www.yeastgenome.org/cgi-bin/GO/goSlimMapper.pl). Sub-cellular compartments are organized on the basis of number of proteins with the indicated annotation, in clockwise falling order.

Figure S3: Relative generation time (h) of deletion mutants. The relative generation time was calculated as (LN [WT/mutant]). Cells were cultured in defined medium in microtiter plates at 30°C and OD was recorded automatically as previously described (69). Medium contained no added NaCl or 0.85 M NaCl. The relative generation time of the salt-sensitive sro7Δ is given as a reference.

Table S1: All proteins identified by iTRAQ analysis

Table S2: Protein abundance in PGV fractions from sec6-4 cells as compared to sro7Δ cells

FilenameFormatSizeDescription
TRA_1186_sm_FigureS1.pdf610KSupporting info item
TRA_1186_sm_FigureS2.pdf44KSupporting info item
TRA_1186_sm_FigureS3.pdf49KSupporting info item
TRA_1186_sm_Tables.doc346KSupporting info item

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