Quantitative Proteomics of Yeast Post-Golgi Vesicles Reveals a Discriminating Role for Sro7p in Protein Secretion
Article first published online: 8 APR 2011
© 2011 John Wiley & Sons A/S
Volume 12, Issue 6, pages 740–753, June 2011
How to Cite
Forsmark, A., Rossi, G., Wadskog, I., Brennwald, P., Warringer, J. and Adler, L. (2011), Quantitative Proteomics of Yeast Post-Golgi Vesicles Reveals a Discriminating Role for Sro7p in Protein Secretion. Traffic, 12: 740–753. doi: 10.1111/j.1600-0854.2011.01186.x
- Issue published online: 9 MAY 2011
- Article first published online: 8 APR 2011
- Received 22 April 2010, revised and accepted for publication 1 March 2011, published online 8 April 2011
Additional Supporting Information may be found in the online version of this article:
Figure S1: Western blot showing the distribution of Snc1/2p and Sso1/2p in sorbitol vesicle gradient fractions from the sro7Δ strain. Samples were prepared and treated as in Figure 1 and Materials and Methods. Proteins were resolved by SDS–PAGE and analyzed by Snc1/2p and Sso1/2p immunoblots.
Figure S2: Breakdown by known localization of the 91 proteins identified in Table 2. Annotations are from the SGD yeast GO slim component catalogue (http://www.yeastgenome.org/cgi-bin/GO/goSlimMapper.pl). Sub-cellular compartments are organized on the basis of number of proteins with the indicated annotation, in clockwise falling order.
Figure S3: Relative generation time (h) of deletion mutants. The relative generation time was calculated as (LN [WT/mutant]). Cells were cultured in defined medium in microtiter plates at 30°C and OD was recorded automatically as previously described (69). Medium contained no added NaCl or 0.85 M NaCl. The relative generation time of the salt-sensitive sro7Δ is given as a reference.
Table S1: All proteins identified by iTRAQ analysis
Table S2: Protein abundance in PGV fractions from sec6-4 cells as compared to sro7Δ cells
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