SEARCH

SEARCH BY CITATION

References

  • 1
    Humbel BM, Marti T, Müller M. Improved structural preservation by combining freeze substitution and low temperature embedding. Beitr Elektronenmikroskop Direktabb Oberfl 1983;16:585594.
  • 2
    Tokuyasu KT. A technique for ultracryotomy of cell suspensions and tissues. J Cell Biol 1973;57:551.
  • 3
    van Donselaar E, Posthuma G, Zeuschner D, Humbel BM, Slot JW. Immunogold labeling of cryosections from high-pressure frozen cells. Traffic 2007;8:471485.
  • 4
    White DL, Andrews SB, Faller JW, Barrnett RJ. The chemical nature of osmium tetroxide fixation and staining of membranes by X-ray photoelectron spectroscopy. Biochim Biophys Acta 1976;436:577592.
  • 5
    Humbel BM. Freeze-substitution. In: CavalierA, SpehnerD, HumbelBM, editors. Handbook of Cryo-Preparation Methods for Electron Microscopy. Boca Raton, Florida, USA: CRC Press; 2009, pp. 319342.
  • 6
    Behrman EJ. The chemistry of osmium tetroxide fixation. In: RevelJP, BarnardT, HaggisGH, editors. The Science of Biological Specimen Preparation. AMF O’Hare, IL: SEM Inc.; 1983, pp. 15.
  • 7
    Dubochet J, Adrian M, Chang JJ, Homo JC, Lepault J, McDowall AW, Schultz P. Cryo-electron microscopy of vitrified specimens. Q Rev Biophys 1988;21:129228.
  • 8
    Dubochet J. The physics of rapid cooling and its implications for cryoimmobilization of cells. Methods Cell Biol 2007;79:721.
  • 9
    Gilkey JC, Staehelin LA. Advances in ultrarapid freezing for the preservation of cellular ultrastructure. J Elec Microsc Tech 1986;3:177210.
  • 10
    Monaghan P, Perusinghe N, Muller M. High-pressure freezing for immunocytochemistry. J Microsc 1998;192:248258.
  • 11
    Dubochet J, Richter K, Roy HV, McDowall AW. Freezing: facts and hypothesis. Scanning Microsc Suppl 1991;5:S1115, S15–16, Discussion.
  • 12
    van Genderen IL, van Meer G, Slot JW, Geuze HJ, Voorhout WF. Subcellular localization of Forssman glycolipid in epithelial MDCK cells by immuno-electron microscopy after freeze-substitution. J Cell Biol 1991;115:10091019.
  • 13
    Agronskaia AV, Valentijn JA, van Driel LF, Schneijdenberg CTWM, Humbel BM, van Bergen en Henegouwen PMP, Verkleij AJ, Koster AJ, Gerritsen HC. Integrated fluorescence and transmission electron microscopy. J Struct Biol 2008;164:183189.
  • 14
    Karreman MA, Agronskaia AV, Verkleij AJ, Cremers FFM, Gerritsen HC, Humbel BM. Discovery of a new RNA-containing nuclear structure in UVC-induced apoptotic cells by integrated laser electron microscopy. Biol Cell 2009;101:287299.
  • 15
    Liou W, Geuze HJ, Slot JW. Improving structural integrity of cryosections for immunogold labeling. Histochem Cell Biol 1996;106:4158.
  • 16
    Jiménez N, Vocking K, van Donselaar EG, Humbel BM, Post JA, Verkleij AJ. Tannic acid-mediated osmium impregnation after freeze-substitution: a strategy to enhance membrane contrast for electron tomography. J Struct Biol 2009;166:103106.
  • 17
    Studer D, Graber W, Al-Amoudi A, Eggli P. A new approach for cryofixation by high-pressure freezing. J Microsc 2001;203:285294.
  • 18
    Al-Amoudi A, Studer D, Dubochet J. Cutting artefacts and cutting process in vitreous sections for cryo-electron microscopy. J Struct Biol 2005;150:109121.
  • 19
    Pierson J, Fernández JJ, Bos E, Amini S, Gnaegi H, Vos M, Bel B, Adolfsen F, Carrascosa JL, Peters PJ. Improving the technique of vitreous cryo-sectioning for cryo-electron tomography: electrostatic charging for section attachment and implementation of an anti-contamination glove box. J Struct Biol 2010;169:219225.
  • 20
    Weibull C, Christiansson A. Extraction of proteins and membrane lipids during low temperature embedding of biological material for electron microscopy. J Microsc 1986;142:7986.
  • 21
    Griffith JM, Posthuma G. A reliable and convenient method to store ultrathin thawed cryosections prior to immunolabeling. J Histochem Cytochem 2002;50:5762.
  • 22
    Sonnenberg A, van Balen P, Hengeveld T, Kolvenbag G, Van Hoeven R, Hilgers J. Monoclonal antibodies detecting different epitopes on the Forssman glycolipid hapten. J Immunol 1986;137:12641269.