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Figure S1: The effect of warming up and cooling down sections of HPF material in the AFS2. VIS of THP-1 cells were transferred to the AFS at −90°C, and directly fixed following VIS2FIXFS (A) or warmed up to −5°C and cooled down again to −90°C, followed by VIS2FIXFS (B). The VIS2FIXFS of both the conditions was performed simultaneously, in 0.1% OsO4 + 0.2%GA + 0.1% UA in acetone. Subsequently the sections were rehydrated, contrasted and examined in the TEM (see Materials and Methods). Ultrastructural cryo-damage could not be observed in the cytoplasm or in the heterochromatin in either condition. Scalebar represents 500 nm.

TRA_1199_sm_FigS1.jpg3371KSupporting info item

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