These authors contributed equally to the work presented in this paper.
VIS2FIX: A High-Speed Fixation Method for Immuno-Electron Microscopy
Article first published online: 28 APR 2011
© 2011 John Wiley & Sons A/S
Volume 12, Issue 7, pages 806–814, July 2011
How to Cite
Karreman, M. A., van Donselaar, E. G., Gerritsen, H. C., Verrips, C. T. and Verkleij, A. J. (2011), VIS2FIX: A High-Speed Fixation Method for Immuno-Electron Microscopy. Traffic, 12: 806–814. doi: 10.1111/j.1600-0854.2011.01199.x
- Issue published online: 12 JUN 2011
- Article first published online: 28 APR 2011
- Accepted manuscript online: 30 MAR 2011 10:34AM EST
- Received 27 October 2010, revised and accepted for publication 29 March 2011, uncorrected manuscript published online 30 March 2011, published online 29 April 2011
Additional Supporting Information may be found in the online version of this article:
Figure S1: The effect of warming up and cooling down sections of HPF material in the AFS2. VIS of THP-1 cells were transferred to the AFS at −90°C, and directly fixed following VIS2FIXFS (A) or warmed up to −5°C and cooled down again to −90°C, followed by VIS2FIXFS (B). The VIS2FIXFS of both the conditions was performed simultaneously, in 0.1% OsO4 + 0.2%GA + 0.1% UA in acetone. Subsequently the sections were rehydrated, contrasted and examined in the TEM (see Materials and Methods). Ultrastructural cryo-damage could not be observed in the cytoplasm or in the heterochromatin in either condition. Scalebar represents 500 nm.
|TRA_1199_sm_FigS1.jpg||3371K||Supporting info item|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.