A Conserved Archaeal Pathway for Tail-Anchored Membrane Protein Insertion
Version of Record online: 3 JUL 2011
© 2011 John Wiley & Sons A/S
Volume 12, Issue 9, pages 1119–1123, September 2011
How to Cite
Sherrill, J., Mariappan, M., Dominik, P., Hegde, R. S. and Keenan, R. J. (2011), A Conserved Archaeal Pathway for Tail-Anchored Membrane Protein Insertion. Traffic, 12: 1119–1123. doi: 10.1111/j.1600-0854.2011.01229.x
- Issue online: 11 AUG 2011
- Version of Record online: 3 JUL 2011
- Accepted manuscript online: 9 JUN 2011 11:07AM EST
- Received 11 April 2011, revised and accepted for publication 8 June 2011, uncorrected manuscript published online 9 June 2011, published online 3 July 2011
Additional Supporting Information may be found in the online version of this article:
Table S1: Data collection and refinement statistics.
Figure S1: Cross-eyed stereo view of electron density for the Mg2+ADP•AlF4−complex of M. thermautotrophicus TRC40. The refined model is superimposed onto a σA-weighted 2Fo − Fc map calculated at 2.1 Å resolution and contoured at 2σ.
Figure S2: Characterization of the depleted translation extract (PD-RRL). Sec61β was translated in PD-RRL in the presence or absence of recombinant yeast Get3. An aliquot was subjected to chemical cross-linking with DSS to evaluate substrate interactions with 5 ng/µL Get3 (top panel). The remainder was incubated with yRMs and analyzed for insertion by the protease protection assay (bottom panel). PD-RRL lacks all known chaperones that interact with ER-directed TA proteins, and is deficient for insertion; activity was restored by supplementing with physiological levels of TRC40/Get3.
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