SEARCH

SEARCH BY CITATION

Supporting Information

Additional Supporting Information may be found in the online version of this article:

Table S1: Data collection and refinement statistics.

Figure S1: Cross-eyed stereo view of electron density for the Mg2+ADPAlF4complex of M. thermautotrophicus TRC40. The refined model is superimposed onto a σA-weighted 2FoFc map calculated at 2.1 Å resolution and contoured at 2σ.

Figure S2: Characterization of the depleted translation extract (PD-RRL). Sec61β was translated in PD-RRL in the presence or absence of recombinant yeast Get3. An aliquot was subjected to chemical cross-linking with DSS to evaluate substrate interactions with 5 ng/µL Get3 (top panel). The remainder was incubated with yRMs and analyzed for insertion by the protease protection assay (bottom panel). PD-RRL lacks all known chaperones that interact with ER-directed TA proteins, and is deficient for insertion; activity was restored by supplementing with physiological levels of TRC40/Get3.

FilenameFormatSizeDescription
TRA_1229_sm_suppinfo.pdf1565KSupporting info item

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.