Bidirectional Insulin Granule Turnover in the Submembrane Space During K+ Depolarization-Induced Secretion
Version of Record online: 13 JUL 2011
© 2011 John Wiley & Sons A/S
Volume 12, Issue 9, pages 1166–1178, September 2011
How to Cite
Hatlapatka, K., Matz, M., Schumacher, K., Baumann, K. and Rustenbeck, I. (2011), Bidirectional Insulin Granule Turnover in the Submembrane Space During K+ Depolarization-Induced Secretion. Traffic, 12: 1166–1178. doi: 10.1111/j.1600-0854.2011.01231.x
- Issue online: 11 AUG 2011
- Version of Record online: 13 JUL 2011
- Accepted manuscript online: 10 JUN 2011 08:32AM EST
- Received 14 February 2011, revised and accepted for publication 9 June 2011, uncorrected manuscript published online 10 June 2011, published online 13 July 2011
Additional Supporting Information may be found in the online version of this article:
Movie S1: Total internal reflection fluorescence microscopy (TIRFM) movie of insulin-EGFP-labeled granules in MIN6 cells. The excitation wavelength was 491 nm (Cobolt Calypso), the objective was a Zeiss α-Plan-Fluar (100×, 1.45 NA), the angle of incidence was 68° and the calculated decay constant of the evanescent field was 84 nm. The acquisition rate was 8.3 Hz, exposure time 50 milliseconds. The cells were continuously perifused at a rate of 0.2 mL/min, the temperature was 37.0 ± 0.1°C. Three minutes prior to the acquisition of this sequence, the K+ concentration in the Krebs-Ringer perifusion medium was increased by switching to a medium containing 40 mM K+. The lag time before the perifusion chamber was reached by the medium was 1 min. Multiple transient brightenings indicate exocytotic activity.
Movie S2: Same experiment and same acquisition conditions as shown in Movie S1. In addition to 40 mM K+, 10 µM nifedipine was present in the perifusion medium. The exposure time of the MIN6 cells to nifedipine prior to the acquisition of the sequence was 5 min.
|TRA_1231_sm_SuppMovie1.mov||835K||Supporting info item|
|TRA_1231_sm_SuppMovie2.mov||612K||Supporting info item|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.