The Interfering Effect of Human IgG Antisperm Antibodies on Human Sperm Penetration of Zona-Free Hamster Eggs

Authors

  • GILBERT G. HAAS JR. MD,

    Corresponding author
    1. Division of Reproductive Biology, Department of Obstetrics and Gynecology, Hospital of the University of Pennsylvania, Philadelphia
      Dept. of Obstetrics and Gynecology, Hospital of the University of Pennsylvania, 106 Dulles Building, 3400 Spruce St, Philadelphia, PA 19104.
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  • JOSEPH E. SOKOLOSKI,

    1. Division of Reproductive Biology, Department of Obstetrics and Gynecology, Hospital of the University of Pennsylvania, Philadelphia
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  • DON P. WOLF

    1. Division of Reproductive Biology, Department of Obstetrics and Gynecology, Hospital of the University of Pennsylvania, Philadelphia
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  • Supported in part by grants from the Andrew Mellon Foundation and NIH Grant HD-06274.

Dept. of Obstetrics and Gynecology, Hospital of the University of Pennsylvania, 106 Dulles Building, 3400 Spruce St, Philadelphia, PA 19104.

Abstract

ABSTRACT: A liquid-phase radioimmunoassay was used to quantitate sperm-associated IgG in a population of patients with unexplained infertility. Plasma IgG antisperm antibody activity was identified in a subpopulation of these patients. Using the human sperm/hamster egg assay, a unique functional test of human sperm penetrating capacity, we identified a subset of these positive patients whose plasma significantly and reproducibly inhibited penetration. This study implies that antigen-antibody reactions may prevent sperm responses that are critical to sperm-egg fusion.

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