Human Soluble Antigens and Their Use in Sperm Antibody Testing

Authors

  • Dr. SIDNEY SHULMAN,

    Corresponding author
    1. Sperm Antibody Laboratory, Departments of Obstetrics and Gynecology and Microbiology, New York Medical College
      Address reprint requests to Sidney Shulman, Sperm Antibody Laboratory, Research Center, Metropolitan Hospital, 1900 Second Avenue, New York, NY 10029.
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  • THOMAS KEANE

    1. Sperm Antibody Laboratory, Departments of Obstetrics and Gynecology and Microbiology, New York Medical College
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  • Supported by the Program tor Applied Research in Fertility Regulation, PARFR P-15. AlDcsd-3608.

Address reprint requests to Sidney Shulman, Sperm Antibody Laboratory, Research Center, Metropolitan Hospital, 1900 Second Avenue, New York, NY 10029.

Abstract

ABSTRACT: Efforts were made to disrupt and solubilize human sperm cells and to evaluate the product for its content of infertility antibody-related antigens. In the procedure that was developed, a well-washed sperm sample is treated with 0.1 M dithiothreitol for 60 min, followed by trypsin at 0.1 mg/ml for 30 min, and then by soybean trypsin inhibitor. A mixture of DNAses I and II are added. After centrifuging, the resuspended pellet (RP) and the final supernatant (FS) show several degrees of cellular breakdown. Immunological evaluation was done with a strongly positive human serum containing sperm-head antibody. From the inhibition of sperm agglutination, we could conclude that the desired soluble antigen was obtained in the FS fraction.

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