Genetic Regulation of Antibody Response to Sheep Red Blood Cells: Isoelectric Focusing Analysis of Sera of Well Responding Strain A/J and Poorly Responding Strain B10 Mice
Version of Record online: 9 MAY 2013
American Journal of Reproductive Immunology
Volume 1, Issue 4, pages 164–167, August 1981
How to Cite
ŘÍHOVÁ, B. and ŘÍHA, I. (1981), Genetic Regulation of Antibody Response to Sheep Red Blood Cells: Isoelectric Focusing Analysis of Sera of Well Responding Strain A/J and Poorly Responding Strain B10 Mice. American Journal of Reproductive Immunology, 1: 164–167. doi: 10.1111/j.1600-0897.1981.tb00029.x
- Issue online: 9 MAY 2013
- Version of Record online: 9 MAY 2013
- Accepted March 3, 1981
- Genetic regulation;
- isoelectric focusing;
- antibody heterogeneity;
- endotoxin stimulation
ABSTRACT: Isoelectric focusing (IEF) on a thin layer of polyacrylamide gel revealed that strain A/J produces antibodies with pI values distributed over the whole assayed pH range (pH 5.05–7.75), while strain B10 produces antibodies of a limited heterogeneity with pI values distributed between pH 6.3 and 7.5. The analysis of sera of congenic resistant (CR) and recombinant strains showed that haplotype H-2a is associated with wide-heterogeneity antibodies, while the presence of haplotype H-2b is associated with the occurrence of limited-heterogeneity antibodies. Thus a gene located within the I region of the major histocompatibility complex (MHC) may affect the degree of heterogeneity of the anti-sheep red blood cell (SRBC) response. The gene is transmitted to the F1 generation as a dominant trait.
After administration of endotoxin (LPS LT2, 10 μg/mouse) to the B10 mice, antibodies with pI values below pH 6.3 and above 7.5 appear in the serum; the heterogeneity of these antibodies resembles that of the A/J strain spectrotype.