Genetic Regulation of Antibody Response to Sheep Red Blood Cells: Linkage to H-2 Complex
Article first published online: 9 MAY 2013
American Journal of Reproductive Immunology
Volume 1, Issue 4, pages 168–173, August 1981
How to Cite
ŘÍHOVÁ, B. and ŘÍHA, I. (1981), Genetic Regulation of Antibody Response to Sheep Red Blood Cells: Linkage to H-2 Complex. American Journal of Reproductive Immunology, 1: 168–173. doi: 10.1111/j.1600-0897.1981.tb00030.x
- Issue published online: 9 MAY 2013
- Article first published online: 9 MAY 2013
- Accepted March 10, 1981
- Genetic regulation;
- immune response to SRBC;
- linkage to MHC;
- IgM-IgG switch;
- inbred mice
ABSTRACT: The analysis of anti-sheep red blood cell (SRBC) antibody production in the congenic resistant (CR) mouse strains A/J and A.BY, B10 and B10.A showed that the level of IgG antibodies after immunization with SRBC is controlled by a gene(s) localized in the H-2 complex. The use of the h2, h4, and i5 H-2 recombinant haplotypes allowed us to map this gene into a region proximally defined by subregion I-J and distally by region H-2G. The IgG antibody level is simultaneously under the influence of non-H-2 genes, of which those of the A/J strain origin determine the high IgG level and those of the B10 strain origin determine the low IgG level.
Weights of spleens of the A/J and B10 mice before and after primary and secondary immunization with SRBC were compared. Before immunization, spleens of the A/J mice were approximately 10% lighter than those of the B10 mice (0.08 g vs 0.09 g). After the first immunization the spleen weights equalized (θ = 0.12 g). Four days after the second immunization the weight of the spleens of A/J mice increased by 312% (relative to the nonimmune state) while that of the B10 mice increased by only 74%. These findings indicate that the cell antigen-specific proliferation in spleens of A/J mice was considerably higher than that in spleens of B10 mice.