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Keywords:

  • Anti-LHRH antibodies;
  • peptide-protein conjugation

ABSTRACT: Antibody response to the decapeptide luteinizing hormone-releasing hormone (LHRH) was induced in mice without use of Freund's complete adjuvant. The conventionally employed conjugates of LHRH with bovine serum albumin were poor immunogens and required potent adjuvants. In contrast, LHRH linked covalently to tetanus toxoid or keyhole limpet hemocyanin-generated antibodies with alum as adjuvant. Methods are described for preparation of LHRH conjugates with these carrier proteins with defined molar composition.