Prof Talwar is a Jawaharlal Nehru Fellow. This work was supported by grants from International Development Research Centre, Canada; the Rockefeller Foundation; the Family Planning Foundation of India; and by the International Committee for Contraception Research of the Population Council, New York, in its contraceptive development program cosponsored by the Ford Foundation and the George J. Hecht Fund. The content does not necessarily reflect the policy of any of the funding sources.
Important Role of the Carrier in the Induction of Antibody Response Without Freund's Complete Adjuvant Against a “Self” Peptide Luteinizing Hormone-Releasing Hormone (LHRH)*
Version of Record online: 9 MAY 2013
American Journal of Reproductive Immunology
Volume 1, Issue 5, pages 262–265, October 1981
How to Cite
SHASTRI, N., MANHAR, S. K. and TALWAR, G. P. (1981), Important Role of the Carrier in the Induction of Antibody Response Without Freund's Complete Adjuvant Against a “Self” Peptide Luteinizing Hormone-Releasing Hormone (LHRH). American Journal of Reproductive Immunology, 1: 262–265. doi: 10.1111/j.1600-0897.1981.tb00051.x
- Issue online: 9 MAY 2013
- Version of Record online: 9 MAY 2013
- Accepted May 6, 1981
- Anti-LHRH antibodies;
- peptide-protein conjugation
ABSTRACT: Antibody response to the decapeptide luteinizing hormone-releasing hormone (LHRH) was induced in mice without use of Freund's complete adjuvant. The conventionally employed conjugates of LHRH with bovine serum albumin were poor immunogens and required potent adjuvants. In contrast, LHRH linked covalently to tetanus toxoid or keyhole limpet hemocyanin-generated antibodies with alum as adjuvant. Methods are described for preparation of LHRH conjugates with these carrier proteins with defined molar composition.