The Molecular Weights and Molecular Weight Heterodispersion of Some Antigens From the Trichloroacetic Acid Soluble Fraction of Human Seminal Plasma
Article first published online: 9 MAY 2013
American Journal of Reproductive Immunology
Volume 1, Issue 5, pages 266–268, October 1981
How to Cite
DE CEREZO, J. M. S. and CEREZO, A. S. (1981), The Molecular Weights and Molecular Weight Heterodispersion of Some Antigens From the Trichloroacetic Acid Soluble Fraction of Human Seminal Plasma. American Journal of Reproductive Immunology, 1: 266–268. doi: 10.1111/j.1600-0897.1981.tb00052.x
- Issue published online: 9 MAY 2013
- Article first published online: 9 MAY 2013
- Accepted April 22, 1981
- Molecular weight heterodispersion;
- trichloroacetic soluble fraction;
- human seminal plasma
ABSTRACT: The antigens isolated from the trichloroacetic acid soluble fraction of human seminal plasma show a polymodal molecular weight heterodispersion. The molecular weights corresponding to the modes of the distribution have limiting values of 2,500-57,000.
Previous results obtained by polyacrylamide gel and cellogel electrophoresis, immunodiffusion and Immunoelectrophoresis, and ultracentrifugation analysis, which suggested homogeneity, were explained by the formation of molecular aggregates, which would also explain why the smaller fragments were not lost during the dialysis carried out in the preparation and purification of the samples.
The average molecular weight of the antigens, determined by gel permeation chromatography and using the equation of Svedberg, is in the range of 10,000–40,000.
It is suggested that the polymodal heterodispersion together with the associating-dissociating properties of these products is used to control their immunological behavior.