Modulation of Endometrial Lymphocyte Response in Menstrual Cycle of Fertile Women

Authors

  • YIGAL SOFFER,

    Corresponding author
    1. Department of Obstetrics and Gynecology and Department of Hematology, Assaf Harofe Hospital, Zerifin
    2. Department of Hormone Research, The Weizmann Institute of Science, Rehovot, Israel
    Search for more papers by this author
  • ELIAHU CASPI,

    1. Department of Obstetrics and Gynecology and Department of Hematology, Assaf Harofe Hospital, Zerifin
    2. Department of Hormone Research, The Weizmann Institute of Science, Rehovot, Israel
    Search for more papers by this author
  • SHOSHANA PELLER,

    1. Department of Obstetrics and Gynecology and Department of Hematology, Assaf Harofe Hospital, Zerifin
    2. Department of Hormone Research, The Weizmann Institute of Science, Rehovot, Israel
    Search for more papers by this author
  • YACOB WEINSTEIN

    1. Department of Obstetrics and Gynecology and Department of Hematology, Assaf Harofe Hospital, Zerifin
    2. Department of Hormone Research, The Weizmann Institute of Science, Rehovot, Israel
    Search for more papers by this author

  • Supported by grant No. 470 from the Ministry of Health, State of Israel, Chief Scientist's Office, to Drs. Soffer and Caspi.

Department of Obstetrics and Gynecology, Assaf Harofe Hospital, Zerifin 70300, Israel.

Abstract

ABSTRACT: Endometrial lymphocytes were isolated in two groups of fertile women, 17 of them normal and seven with various clinical problems. In order to study their immunological properties, endometrial and blood lymphocytes were cultured with either phytohemagglutinin (PHA), concanavalin A (Con A), pokeweed (PW), or purified protein derivative (PPD). Lymphocyte response was assayed by tritiated thymidine incorporation. The percent of T cells was estimated by E-rosette formation. In the normal fertile group, endometrial lymphocyte response to PHA and to Con A was found to correlate with the days of menstrual cycle, rising in a linear fashion between days 9 and 24. In the group with clinical problems, no correlation was noted. In neither group were endometrial lymphocyte responses to PW or PPD or percent of endometrial lymphocyte rosette formation influenced by the menstrual cycle. The modulation of endometrial lymphocyte response to PHA or to Con A stimulation in normal fertile women during the menstrual cycle seems unrelated to the number of T cells.

Ancillary