Problems in the Measurement of Putative Serum Immune Complexes by the Method of Beaumont

Authors


Department of Obstetrics & Gynecology, Baylor College of Medicine, Houston TX 77030.

Abstract

ABSTRACT: A methodological investigation of the procedure used by Beaumont et al for measuring a “serum immune complex” precipitated by 25% ammonium sulfate and alleged to contain an ethynyl-estradiol binding immunoglobulin has found major problems with reproducibility and with the correlation of total protein as measured by the Lowry method and the IgG content as determined by a specific nephelometric procedure.

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