Problems in the Measurement of Putative Serum Immune Complexes by the Method of Beaumont


Department of Obstetrics & Gynecology, Baylor College of Medicine, Houston TX 77030.


ABSTRACT: A methodological investigation of the procedure used by Beaumont et al for measuring a “serum immune complex” precipitated by 25% ammonium sulfate and alleged to contain an ethynyl-estradiol binding immunoglobulin has found major problems with reproducibility and with the correlation of total protein as measured by the Lowry method and the IgG content as determined by a specific nephelometric procedure.