This work was supported by a grant from the Fonds der Österreichischen Krebs-forschungsinstitute.
Monoclonal Antibodies Against Human Chorionic Gonadotropin (hCG): I. Production, Specificity, and Intramolecular Binding Sites*
Article first published online: 9 MAY 2013
American Journal of Reproductive Immunology
Volume 2, Issue 4, pages 212–216, August 1982
How to Cite
KOFLER, R., BERGER, P. and WICK, G. (1982), Monoclonal Antibodies Against Human Chorionic Gonadotropin (hCG): I. Production, Specificity, and Intramolecular Binding Sites. American Journal of Reproductive Immunology, 2: 212–216. doi: 10.1111/j.1600-0897.1982.tb00168.x
- Issue published online: 9 MAY 2013
- Article first published online: 9 MAY 2013
- Accepted March 24, 1982
- Monoclonal antibody;
- human chorionic gonadotropin;
- human luteinizing hormone;
- human follicle-stimulating hormone;
- human thyroid-stimulating hormone;
ABSTRACT: Thirty-nine monoclonal antibody (MCA) producing hybridoma cell lines derived from fusions of mouse myeloma cells with spleen cells from mice immunized with human chorionic gonadotropin (hCG) have been established. Their products have been tested in radioimmunoassays using 125I-labeled hCG, luteinizing hormone (LH), follicle-stimulating hormone (FSH), thyroid-stimulating hormone (TSH), the alpha (α) and beta (β) subunits of hCG and LH, and the C-terminal peptide 109–145 (CTP) of CG. All MCA were, in addition, tested in indirect immunofluorescence (IIF) on paraffin sections of human pituitary glands.
According to the intramolecular localization of the determinants recognized, three main groups of MCA can be distinguished: 1) MCA directed against epitopes on the β-chain (α-MCA), 2) MCA directed against (β-chain determinants (β-MCA), and 3) MCA reacting with a conformational determinant only present on the native hormone and not on either subunit (con-formational-MCA). All α-MCA cross-react with human LH, FSH, and TSH. The β-MCA do not react with FSH or TSH, but do react to a varying degree with LH. The conformational-MCA show no binding of labeled FSH or TSH and very little or no cross-reactivity with LH.