This was supported in part by a grant from the National Cancer Institute, 1-R01-CA23533-04, and in part by grants from the Gaston County Cancer Society and Forsyth Cancer Service.
“Membrane-Associated” Immunoglobulins in Cyst and Ascites Fluids of Ovarian Cancer Patients*
Article first published online: 9 MAY 2013
American Journal of Reproductive Immunology
Volume 3, Issue 1, pages 7–11, January 1983
How to Cite
TAYLOR, D. D., HOMESLEY, H. D. and DOELLGAST, G. J. (1983), “Membrane-Associated” Immunoglobulins in Cyst and Ascites Fluids of Ovarian Cancer Patients. American Journal of Reproductive Immunology, 3: 7–11. doi: 10.1111/j.1600-0897.1983.tb00204.x
- Issue published online: 9 MAY 2013
- Article first published online: 9 MAY 2013
- Accepted July 28, 1982
- Antitumor antibodies
ABSTRACT: A new column chromatography technique, utilizing peroxidase-labeled antihuman immunoglobulins, is described, which can measure the binding of specific antibodies to membrane fragments containing relevant antigens. Using this assay, the presence of bound immunoglobulin is demonstrated on membrane fragments isolated from ascites fluids, but not from tumor tissues. In this study, two populations of immunoglobulins were examined: ‘free’ immunoglobulins and immunoglobulins bound to ascites fluid-derived membrane fragments. Immunoglobulins eluted from membrane fragments of the ascites fluids were capable of binding to the tumor. These were specific for binding to tumor membrane preparations, in that there was two orders of magnitude greater binding of these immunoglobulins at a given concentration than ‘free’ immunoglobulins at the same concentration.