Isolation of Rat Male Accessory Glands Autoantigens by Affinity Chromatography


  • This work was supported by the Consejo Nacional de Investigaciones Cientificas y Técnicas, Argentina. Dr. Pesoa and Dr. Pistoresi-Palencia are recipients of scholarships from the same organization.

Cátedra de Inmunología y Serología, Laboratorio Central, Hospital Nacional de Clínicas, Santa Rosa 1564, 5000 CORDOBA, Argentina.


ABSTRACT: The autoantigens of rat male accessory glands were isolated by a short procedure which involved 1) immunization of rats with chemically modified rat male accessory glands' saline extract; 2) purification of immunoglobulin G (IgG) from the autoantisera by chromatography on DEAE-Sephadex A-50; 3) coupling of rat IgG anti-rat male accessory glands with 4-B activated Sepharose; 4) addition of rat male accessory glands' saline extract and removal of autoantigens by glycine-HCl, pH 2.9. The purity of the eluted autoantigens was determined by polyacrylamide disc gel electrophoresis (PAGE). These components retained their immunologic activity as demonstrated by inhibition of tanned cell hemagglutination and double immunodiffusion gel precipitation.