This work was supported by the Consejo Nacional de Investigaciones Cientificas y Técnicas, Argentina. Dr. Pesoa and Dr. Pistoresi-Palencia are recipients of scholarships from the same organization.
Isolation of Rat Male Accessory Glands Autoantigens by Affinity Chromatography*
Article first published online: 9 MAY 2013
American Journal of Reproductive Immunology
Volume 4, Issue 2, pages 67–70, September 1983
How to Cite
PESOA, S. A., PISTORESI-PALENCIA, M. C., GALMARINI, M., VOTTERO-CIMA, E. and RIERA, C. M. (1983), Isolation of Rat Male Accessory Glands Autoantigens by Affinity Chromatography. American Journal of Reproductive Immunology, 4: 67–70. doi: 10.1111/j.1600-0897.1983.tb00255.x
- Issue published online: 9 MAY 2013
- Article first published online: 9 MAY 2013
- Accepted January 27, 1983
- Autoantigen's purification;
- male accessory glands;
ABSTRACT: The autoantigens of rat male accessory glands were isolated by a short procedure which involved 1) immunization of rats with chemically modified rat male accessory glands' saline extract; 2) purification of immunoglobulin G (IgG) from the autoantisera by chromatography on DEAE-Sephadex A-50; 3) coupling of rat IgG anti-rat male accessory glands with 4-B activated Sepharose; 4) addition of rat male accessory glands' saline extract and removal of autoantigens by glycine-HCl, pH 2.9. The purity of the eluted autoantigens was determined by polyacrylamide disc gel electrophoresis (PAGE). These components retained their immunologic activity as demonstrated by inhibition of tanned cell hemagglutination and double immunodiffusion gel precipitation.