Incidence of Neutrophil Antigens on Human Cord Neutrophils

Authors

  • Prema R. Madyastha PhD,

    Corresponding author
    1. Departments of Laboratory Medicine and Pediatrics, Medical University of South Carolina, Charleston, South Carolina
      Department of Laboratory Medicine, Medical University of South Carolina, 171 Ashley Avenue, Charleston, SC 29425.
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  • Armand B. Glassman,

    1. Departments of Laboratory Medicine and Pediatrics, Medical University of South Carolina, Charleston, South Carolina
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  • David H. Levine

    1. Departments of Laboratory Medicine and Pediatrics, Medical University of South Carolina, Charleston, South Carolina
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Department of Laboratory Medicine, Medical University of South Carolina, 171 Ashley Avenue, Charleston, SC 29425.

Abstract

ABSTRACT: Neutrophils isolated from cord blood of healthy newborns (33 blacks and 21 whites) were investigated by EDTA-microagglutination for their expression of neutrophil specific antigens that have been associated with isoimmune neonatal or autoimmune neutropenia. Equal volumes of various neutrophil anti-sera (2 μ) and cord neutrophils (3–5 × 106/ml) were mixed in tissue typing microplates under oil and were incubated at room temperature for 6–8 hr, following which the degree of agglutination was noted. Our data revealed that all the currently recognized neutrophil antigens are readily demonstrable by antineutrophil antibodies in cord blood, (NA1, 52–54%; NA2, 81–85%; NB1, 95–96%; NC1, 90%; 9A, 29–30%) suggesting that neutrophil antigens are fully expressed at birth.

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