In Vitro Release of Pregnancy-Associated Plasma Protein-A (PAPP-A) by Human Endometrial Cells
Article first published online: 9 MAY 2013
American Journal of Reproductive Immunology and Microbiology
Volume 10, Issue 4, pages 139–142, April 1986
How to Cite
BISCHOF, P. and TSENG, L. (1986), In Vitro Release of Pregnancy-Associated Plasma Protein-A (PAPP-A) by Human Endometrial Cells. American Journal of Reproductive Immunology and Microbiology, 10: 139–142. doi: 10.1111/j.1600-0897.1986.tb00013.x
- Issue published online: 9 MAY 2013
- Article first published online: 9 MAY 2013
- Accepted December 13, 1985
- Endometrial cells;
ABSTRACT: Evidence has now accumulated to sustain the idea that pregnancy-associated plasma protein-A (PAPP-A) is not pregnancy specific: it can be localized immunohistochemically in nonpregnant human endometrium. Thus, it was of interest to see if a primary cell culture of human endometrial cells was capable of producing PAPP-A.
Immunoreactive PAPP-A-like material was detected in the culture medium of glandular as well as stromal cells. The presence of PAPP-A in endometrial culture medium was specific and could not be attributed to a nonspecific protease effect on the tracer used in radioimmunoassay. The production rate of PAPP-A by stromal cells could be enhanced by the addition of medroxyprogesterone acetate (MPA). Estradiol (E2) alone or in combination with MPA did not modify the production rate of PAPP-A. The production rate of PAPP-A by glandular cells remained unchanged after treatment with MPA and/or E2. These results confirm that in nonpregnant women PAPP-A is a progesterone-dependent protein produced by the endometrium.