Carbohydrates of the Surface of the Normal Human Spermatozoon

Authors

  • MARÍA N. MAZZINI,

    1. Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
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  • PABLO CERACI,

    1. Centro de Investigationes en Reproducción, Facultad de Medicina, Universidad de Buenos Aires, Argentina
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  • JOSEFINA M.S. DE CEREZO,

    Corresponding author
    1. Centro de Investigationes en Reproducción, Facultad de Medicina, Universidad de Buenos Aires, Argentina
      Centro de Investigaciones en Reproduccíon, Facultad de Medicina, Piso 10, 1121 Buenos Aires, Argentina.
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  • ALBERTO S. CEREZO

    1. Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
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Centro de Investigaciones en Reproduccíon, Facultad de Medicina, Piso 10, 1121 Buenos Aires, Argentina.

Abstract

ABSTRACT: Autoproteolysis of human spermatozoa produces oligopeptides with oligosaccharide chains of the N-glycosidic-linked type that are released from the “surface exposed” parts of glycoproteins. The products eluted in the previous washing of the spermatozoa have the same composition and solubility characteristics as the oligopeptides from the digestion. This suggests that autoproteolysis is a constant process that normally occurs on the spermatozoa membrane. The cytochemical characterization and localization of the N-glycosidic-linked oligosaccharide receptors on the human spermatozoa membrane after digestion, in the presence or absence of seminal plasma, indicates that only part of the oligosaccharides are cleaved. Their distribution on the different zones of the spermatozoon changed as the probability of detecting these receptors in the intermediate segment increased after proteolysis; this indicates that in this zone the receptors are cryptic ones that become exposed by the action of the proteolytic enzymes. In the presence of seminal plasma most receptors on the acrosome are eliminated.

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