Graft-vs-Host Reaction Induced by Para-aortic Lymph Nodes During Estrous and Diestrous Stages

Authors

  • YOLANDA P. DE BONAPARTE,

    Corresponding author
    1. Experimental Cancer and Animal Care Laboratory, Department of Investigation, Instituto de Oncología “Angel H. Roffo,” Facultad de Medicina, UBA, Buenos Aires, Argentina
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  • LUCAS L. COLOMBO,

    1. Experimental Cancer and Animal Care Laboratory, Department of Investigation, Instituto de Oncología “Angel H. Roffo,” Facultad de Medicina, UBA, Buenos Aires, Argentina
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    • Lucas L. Colombo is a fellow of the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET).

  • SLOBODANKA KLEIN,

    1. Experimental Cancer and Animal Care Laboratory, Department of Investigation, Instituto de Oncología “Angel H. Roffo,” Facultad de Medicina, UBA, Buenos Aires, Argentina
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  • ISABEL S. D'ELIA,

    1. Experimental Cancer and Animal Care Laboratory, Department of Investigation, Instituto de Oncología “Angel H. Roffo,” Facultad de Medicina, UBA, Buenos Aires, Argentina
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  • MIRIAM DIAMENT

    1. Experimental Cancer and Animal Care Laboratory, Department of Investigation, Instituto de Oncología “Angel H. Roffo,” Facultad de Medicina, UBA, Buenos Aires, Argentina
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Instituto de Oncología “Angel H. Roffo,” Av. San Martín 5481, Buenos Aires, 1417, Argentina.

Abstract

ABSTRACT: Mouse uterine draining lymph node (UDLN) cells, or soluble factors released by them in both phases of the estrous cycle were examined in the graft-vs-host (GVH) assay. The immune response during the estrous phase was significantly decreased as compared with diestrous phase; 24-hour culture supernatant of UDLN cells was also able to decrease GVH activity mounted in F1 hybrids. This difference in reactivity was not found when peripheral nodes (PN) or spleen cells were tested. These results show that in physiological conditions it is possible to detect an hormonal influence on T lymphocyte function and would suggest that a nonspecific immunosuppression would appear even during the estrous phase.

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