Phosphate Content of Human Amniotic Fluid and Its Relationship to Bacterial Growth Inhibition

Authors

  • JAMES TOMBLIN,

    1. Department of Obstetrics and Gynecology, Marshall University School of Medicine, Huntington, West Virginia
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    • James Tomblin was aided by the Summer Science Research Program For Medical Students grant 8–83-81 from the March of Dimes Birth Defects Foundation. He is currently a resident in Obstetrics and Gynecology at Ohio State University, Columbus, Ohio.

  • B. DAVIS,

    1. Department of Obstetrics and Gynecology, Marshall University School of Medicine, Huntington, West Virginia
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  • Dr. B. LARSEN

    Corresponding author
    1. Department of Microbiology, Marshall University School of Medicine, Huntington, West Virginia
    2. Department of Obstetrics and Gynecology, Marshall University School of Medicine, Huntington, West Virginia
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Department of Obstetrics and Gynecology, Marshall University School of Medicine, 1801 6th Avenue, Huntington, WV 25701.

Abstract

ABSTRACT: A sensitive method for assay of phosphate has been adapted for use with human amniotic fluid. The presence of an antibacterial factor in amniotic fluid inactivated by phosphate suggested that measurement of free phosphate may predict antibacterial activity and hence susceptibility to intrapartum infection. The intrinsic antibacterial activity of amniotic fluid was altered by addition of large amounts of phosphate, but the intrinsic concentration of free phosphate correlated poorly with antibacterial activity and organically combined phosphates did not appear to be related to the antibacterial property.

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