Antigenic and Cytochemical Characterization of Cells Adhering to Intrauterine Contraceptive Devices
Article first published online: 9 MAY 2013
American Journal of Reproductive Immunology and Microbiology
Volume 13, Issue 3, pages 83–86, March 1987
How to Cite
ENNAS, M. G., LACONI, S., MANCONI, P. E., MARCELLO, C., PARODO, G., PULIGHEDDU, P. and LECCA, U. (1987), Antigenic and Cytochemical Characterization of Cells Adhering to Intrauterine Contraceptive Devices. American Journal of Reproductive Immunology and Microbiology, 13: 83–86. doi: 10.1111/j.1600-0897.1987.tb00098.x
- Issue published online: 9 MAY 2013
- Article first published online: 9 MAY 2013
- Accepted December 23, 1986
- monoclonal antibodies;
ABSTRACT: Eighteen copper intrauterine devices (IUDs), removed after 25 months of use, were examined to evaluate cells adhering to them (IAC). By means of monoclonal antibodies, the antigenic phenotype of IAC was studied, along with some IAC cytochemical properties. Sixty percent of IAC were identified as granulocytes based on morphological, cytochemical, and antigenic characteristics. A small proportion of IAC were shaped like large foreign-body macrophages, with multiple picnotic nuclei, and diffused alpha naphtyl acetate esterase (ANAE) activity. Some IAC identified as macrophages from a morphological view point also showed dipeptidil-diaminopeptidase IV (DAPIV) reactivity, previously described only in T-helper lymphocytes. Most IAC identified as macrophages reacted with the monoclonal antibodies OKM1 and HLA-DR, and showed ANAE activity in the form of small multiple granules.
The hypothesis that IUD-adhering macrophages with an ANAE +, DAPIV+, OKM1 +, and HLA-DR + phenotype could play a role in the inactivation of spermatozoa can be proposed.