Antigen Presenting Cells in Human Decidual Tissue: III. Role of Accessory Cells in the Activation of Suppressor Cells

Authors

  • JORGE R. OKSENBERG,

    1. The Lautenberg Center for Tumor and General Immunology, Hadassah Medical School, the Hebrew University of Jerusalem
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  • SCHLOMO MOR-YOSEF,

    1. The Lautenberg Center for Tumor and General Immunology, Hadassah Medical School, the Hebrew University of Jerusalem
    2. Department of Obstetrics and Gynecology, Hadassah University Hospital), and Tissue Typing Unit
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  • YOSEF EZRA,

    1. The Lautenberg Center for Tumor and General Immunology, Hadassah Medical School, the Hebrew University of Jerusalem
    2. Department of Obstetrics and Gynecology, Hadassah University Hospital), and Tissue Typing Unit
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  • Professor CHAIM BRAUTBAR

    Corresponding author
    1. The Lautenberg Center for Tumor and General Immunology, Hadassah Medical School, the Hebrew University of Jerusalem
    2. Department of Clinical Microbiology, Hadassah University Hospital, Jerusalem, Israel
      Tissue Typing Unit, Hadassah University Hospital, Kiryat Hadassah, Jerusalem, Israel.
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Tissue Typing Unit, Hadassah University Hospital, Kiryat Hadassah, Jerusalem, Israel.

Abstract

ABSTRACT: Human decidual antigen presenting cells (DAPCs) exposed to fetal cells in vitro induce generation of suppressor T cells among a population of peripheral blood lymphocytes. Human lymphocyte antigen (HLA)-class II positive antigen presenting cells were isolated from early normal human decidual tissue and from peripheral blood (PAPCs) by adhering Ficoll-Pa-que separated cell suspensions to fibronectin. In contrast to PAPCs, DAPCs pulsed with fetal antigens induced a radio-sensitive, Leu 1,2-positive T suppressor cell population. A nylon wool adherent B cell population is required during the in vitro induction of the suppressor cells. These suppressor cells impair primary mitogen and mixed lymphocyte culture (MLC) responses, generation of anti-trinitrophe-nyl (TNP) cytotoxic T lymphocytes, and antibody response of autologous and allogeneic lymphocytes. Only intact viable embryonic cells can effectively confer upon DAPCs the ability to induce T suppressor cells. The T suppressor cell induction by DAPCs primed with fetal antigens is restricted by the major histocompatibility complex. Our results show that the HLA-DR molecules are the most prominent restriction elements.

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