ABSTRACT: The presence of autoantibodies in the fetus has previously been investigated in the offspring of mothers with autoimmune diseases, but not in the offspring of healthy pregnant women. Employing the ELISA method, we examine four SLE-associated autoantibodies (anti-dsDNA, anti-ssDNA, anti-poly(I), and anti-cardiolipin) in sera obtained from 196 healthy pregnant women and their offspring. All detected autoantibodies in maternal and blood cord sera were of the IgM isotype. Thirty-four maternal sera (17.3%) were positive for one or more tested autoantibodies: 16 (8.1%) for one autoantibody alone, 17 (8.6%) for two autoantibodies, and one (0.5%) for three autoantibodies; 1.5%, 5.6%, 9.6%, and 10.2% of the maternal sera were positive for autoantibodies against dsDNA, ssDNA, poly(I), and cardiolipin, respectively. In only one blood cord serum sample was an autoantibody (IgM anti-ssDNA) detected. This infant was born to a healthy seronegative mother. The finding that all offspring of IgM seropositive mothers are IgM seronegative is not surprising since maternal IgM autoantibodies do not cross the placenta. The finding of an IgM seropositive infant born to an IgM seronegative mother may indicate that the fetus is capable of self-production of autoantibodies.