Membrane Fluidity of Trophoblast Cells and Susceptibility to Natural Cytotoxicity
Article first published online: 9 MAY 2013
American Journal of Reproductive Immunology
Volume 19, Issue 3, pages 92–98, March 1989
How to Cite
SZEKERES-BARTHO, J., NÉMETH, A., VARGA, P., CSERNUS, V., KÖSZEGI, T. and PAÁL, M. (1989), Membrane Fluidity of Trophoblast Cells and Susceptibility to Natural Cytotoxicity. American Journal of Reproductive Immunology, 19: 92–98. doi: 10.1111/j.1600-0897.1989.tb00555.x
- Issue published online: 9 MAY 2013
- Article first published online: 9 MAY 2013
- Accepted January 22, 1989
- Membrane lipid microviscosity;
- cholesteryl hemisuccinate;
- spontaneous abortion;
ABSTRACT: This study examines the relationship between membrane lipid microviscosity and susceptibility of villous trophoblast to lysis by natural cytotoxic cells.
Trophoblast-enriched cell suspensions prepared from term human placentae were treated with cholesteryl hemisuccinate (CHS)—a modulator of membrane lipid microviscosity. CHS-treated cells were more susceptible targets for natural lymphocyte cytotoxicity than were untreated controls. In binding experiments, increased binding of lymphocytes to CHS-treated target cells was found. Preincubation with progesterone prevented membrane rigidification by CHS. Progesterone, Cortisol, and estriol restored the impaired resistance of CHS-treated trophoblast cells to lysis.
We determined microviscosity and progesterone concentration in villous surface membranes, prepared from placentae from idiopathic spontaneous abortions and normal first-trimester pregnancies.
An inverse relationship was found between progesterone content and microviscosity of the membranes. Microviscosity of the membranes from abortion placentae was significantly higher (P < .01) and progesterone concentration was significantly lower (P < .001) than those in the membranes of normal first trimester placentae.