Suppression of T-Lymphocyte Blastogenesis by Ovine Trophoblast Protein-1 and Human Interferon-α May Be Independent of Interleukin-2 Production


158 ASRC, University of Missouri, Columbia, MO 65211.


ABSTRACT: Cells derived from the trophoblast tissue of a day 15 sheep conceptus released substances that inhibit incorporation of [3H]thymidine into phytohemagglutinin (PHA)-stimulated ovine lymphocytes. This effect was partially reversed by addition of antiserum to ovine trophoblast protein-1 (oTP-1), a major secretory product of day 13–21 sheep conceptuses and a protein structurally and functionally related to α-interferons (IFN-α). Human IFN-a, unlike dexamethasone, inhibits phytohemagglutinin-induced lymphocyte blastogenesis without reducing interleukin-2 (IL-2) production by the cultures, and conditioned medium containing IL-2 does not promote [3H]thymidine incorporation into ovine lymphocytes when oTP-1 is present. Thus, oTP-1, by virtue of being an IFN, may have a local immunomodulatory role by selectively inhibiting the proliferative responses of certain maternal immune cells to IL-2.