Bioneutralization Capacity of the Antibodies Generated in Women by the Beta Subunit of Human Chorionic Gonadotropin (βhCG) and βhCG Associated with the Alpha Subunit of Ovine Luteinizing Hormone Linked to Carriers


Immuno-Endocrinology Group, National Institute of Immunology, Shaheed Jeet Singh Marg, New Delhi, India.


ABSTRACT: Data is presented on the bioneutralization capacity per unit immunoactivity of 30 serum samples of women immunized with the β-sub-unit of human chorionic gonadotropin (β-hCG) or βhCG associated noncovalently with the α-sub-unit of ovine luteinizing hormone (α-oLH), to form a heterospecies dimer (HSD), which were linked to carriers. The bioassays utilized were inhibition of radioiodinated hCG binding to rat testicular receptors in vitro and the inhibition of hCG induced testosterone production in mice. In both assays, antisera of women immunized with the HSD had a bioactivity to immunoactivity ratio that was about 25% higher, on an average, than antisera of women immunized with β-hCG, suggesting a better bioneutralization capacity of sera raised by the HSD in women.