Detection of Immunoregulatory Lipid-Like Factors in Human Amniotic Fluid

Authors

  • RICARDO RUEDA,

    1. Sección de Inmunología, Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Hospital Universitario, Universidad de Granada, Granada, Spain
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  • MA LUISA VARGAS,

    1. Sección de Inmunología, Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Hospital Universitario, Universidad de Granada, Granada, Spain
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  • MARCOS GARCÍA-PACHECO,

    1. Sección de Inmunología, Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Hospital Universitario, Universidad de Granada, Granada, Spain
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  • ENRIQUE GARCÍA-OLIVARES

    Corresponding author
    1. Sección de Inmunología, Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Hospital Universitario, Universidad de Granada, Granada, Spain
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Sección de Inmunología, Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Hospital Universitario, Universidad de Granada, 18012 Granada, Spain.

Abstract

ABSTRACT: The immunosuppressive activity of amniotic fluid (AF) is extensively documented in the mouse. Although this property is due in part to the presence of alpha-fetoprotein (α-FP), other immunosuppressive factors are suspected. In this article, we demonstrate that human amniotic fluid lipid extract (AFLE) is inhibitory of, although not cytotoxic to, PHA-activated human lymphocytes, of mouse bone marrow cells, and of different established cell lines of human and mouse source. This effect is shown to be reversible. Under preparative thin layer chromatography (TLC) using chloroform:methanol:water (60:38:8) as solvents, the activity of AFLE migrates to two peaks of inhibition with Rf values of 0.46-0.62 and 0.84-1, respectively. These lipid-like factors may play a role as a nonspecific immunoregulatory mechanism which prevents maternally mediated immune rejection of the conceptus.

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