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Keywords:

  • Estradiol;
  • progesterone

ABSTRACT: The effects of estradiol (E2), progesterone (P), and danazol on the production of interleukin-1β (IL-1β) and tumor necrosis factor (TNF) by OK-432 (a streptococcal preparation)-stimulated monocytes were examined. E2 and P at physiologic concentrations enhanced IL-1β and TNF production by monocytes from donors with lower control levels (without steroids added) of IL-1β and TNF. However, E2 and P at physiologic concentrations did not affect IL-1β and TNF production by monocytes from donors with higher control levels of IL-1β and TNF. Danazol inhibited IL-1β and TNF production by monocytes in a dose-dependent manner from not only donors with lower control levels of IL-1β and TNF but also donors with higher control levels of IL-1β and TNF. Danazol at a concentration of 10−6 M significantly suppressed IL-1β and TNF production in the presence of E2 and/or P at concentrations giving peak responses of IL-1β production. These findings suggest possible new mechanisms of action for danazol in the treatment of endometriosis and infertility associated with immune abnormalities.