Absence of Immunoreactive Cytokines in Supernatants of Individual Preimplantation Human Embryos

Authors

  • DAVID B. SEIFER M.D.,

    Corresponding author
    1. Division of Reproductive Endocrinology, Women and Infants Hospital, Brown University School of Medicine, Providence, Rhode Island
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    • David B. Seifer is a recipient of the Physician-Scientist Award from NIH-NIA (AG00566). Roberto Romero is a recipient of the Physician-Scientist Award from NIH-NICHD.

  • ROBERTO ROMERO,

    1. Department of Obstetrics and Gynecology, Hutzel Hospital, Wayne State University School of Medicine, Detroit. Michigan
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  • DAVID BERLINSKY,

    1. Division of Reproductive Endocrinology, Women and Infants Hospital, Brown University School of Medicine, Providence, Rhode Island
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  • RAY V. HANING JR.

    1. Division of Reproductive Endocrinology, Women and Infants Hospital, Brown University School of Medicine, Providence, Rhode Island
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Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology, Women and Infants Hospital, 101 Dudley Street, Providence, RI 02905.

Abstract

PROBLEM: The purpose of this study was to determine if it was possible to use commercially available enzyme-linked immunosorbent assay (ELISA) kits to quantitate specific cytokine production from individual embryos.

METHODS: We examined the supernatants of individual two to six cell preimplantation human embryos using commerically available kits for IL-1 alpha, IL-1 beta, IL-6, and LIF.

RESULTS: Cytokines were undetectable in all media samples analyzed.

CONCLUSION: While it is possible that individual preimplantation embryos may produce cytokines, we believe these concentrations are below the detectable limits of the commercial assays used in this study.

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