David B. Seifer is a recipient of the Physician-Scientist Award from NIH-NIA (AG00566). Roberto Romero is a recipient of the Physician-Scientist Award from NIH-NICHD.
Absence of Immunoreactive Cytokines in Supernatants of Individual Preimplantation Human Embryos
Article first published online: 9 MAY 2013
American Journal of Reproductive Immunology
Volume 30, Issue 2-3, pages 105–107, September-October 1993
How to Cite
SEIFER, D. B., ROMERO, R., BERLINSKY, D. and HANING, R. V. (1993), Absence of Immunoreactive Cytokines in Supernatants of Individual Preimplantation Human Embryos. American Journal of Reproductive Immunology, 30: 105–107. doi: 10.1111/j.1600-0897.1993.tb00610.x
- Issue published online: 9 MAY 2013
- Article first published online: 9 MAY 2013
- Accepted June 28, 1993
- human preimplantation embryos;
PROBLEM: The purpose of this study was to determine if it was possible to use commercially available enzyme-linked immunosorbent assay (ELISA) kits to quantitate specific cytokine production from individual embryos.
METHODS: We examined the supernatants of individual two to six cell preimplantation human embryos using commerically available kits for IL-1 alpha, IL-1 beta, IL-6, and LIF.
RESULTS: Cytokines were undetectable in all media samples analyzed.
CONCLUSION: While it is possible that individual preimplantation embryos may produce cytokines, we believe these concentrations are below the detectable limits of the commercial assays used in this study.