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Keywords:

  • Cytokines;
  • human preimplantation embryos;
  • ELISA

PROBLEM: The purpose of this study was to determine if it was possible to use commercially available enzyme-linked immunosorbent assay (ELISA) kits to quantitate specific cytokine production from individual embryos.

METHODS: We examined the supernatants of individual two to six cell preimplantation human embryos using commerically available kits for IL-1 alpha, IL-1 beta, IL-6, and LIF.

RESULTS: Cytokines were undetectable in all media samples analyzed.

CONCLUSION: While it is possible that individual preimplantation embryos may produce cytokines, we believe these concentrations are below the detectable limits of the commercial assays used in this study.