PROBLEM: Although chorion produces prostaglandins in response to interleukin-1β (IL-1β), tumor necrosis factor alpha (TNFβ), and epidermal growth factor (EGF), little attention has been given to the mechanisms of action of prostaglandin biosynthesis in this tissue.
METHODS: IL-1β, TNFβ, and EGF induced a concentration-related stimulation of prostaglandin E2 (PGE2) biosynthesis in human chorion cells, and this stimulation was enhanced by the addition of exogenous arachidonic acid.
RESULTS: Protein synthesis inhibition with cycloheximide or actinomycin D resulted in complete abrogation of the stimulation of PGE2 production by IL-1β, TNFα, and EGF. Finally, all three stimulants induced a more rapid recovery of PGE2 production in chorion cells after ace-tylsalicylic acid pretreatment than controls.
CONCLUSIONS: It is suggested that IL-1β, TNFα, and EGF all act to stimulate human chorion PGE2 production primarily via induction of prostaglandin endoperoxide synthase activity.