Evidence for a Common Mechanism of Action of Interleukin-1β, Tumor Necrosis Factor-α, and Epidermal Growth Factor on Prostaglandin Production in Human Chorion Cells


Department of Obstetrics and Gynecology, University of Utah School of Medicine, 50 North Medical Drive, Salt Lake City, UT 84132.


PROBLEM: Although chorion produces prostaglandins in response to interleukin-1β (IL-1β), tumor necrosis factor alpha (TNFβ), and epidermal growth factor (EGF), little attention has been given to the mechanisms of action of prostaglandin biosynthesis in this tissue.

METHODS: IL-1β, TNFβ, and EGF induced a concentration-related stimulation of prostaglandin E2 (PGE2) biosynthesis in human chorion cells, and this stimulation was enhanced by the addition of exogenous arachidonic acid.

RESULTS: Protein synthesis inhibition with cycloheximide or actinomycin D resulted in complete abrogation of the stimulation of PGE2 production by IL-1β, TNFα, and EGF. Finally, all three stimulants induced a more rapid recovery of PGE2 production in chorion cells after ace-tylsalicylic acid pretreatment than controls.

CONCLUSIONS: It is suggested that IL-1β, TNFα, and EGF all act to stimulate human chorion PGE2 production primarily via induction of prostaglandin endoperoxide synthase activity.