Phenotypic Characterization of CD7 +, CD3 +, and CD8+ Lymphocytes From First Trimester Human Decidua Using Two-Color Flow Cytometry
Version of Record online: 9 MAY 2013
American Journal of Reproductive Immunology
Volume 29, Issue 1, pages 5–16, January 1993
How to Cite
CHERNYSHOV, V. P., SLUKVIN, I. I. and BONDARENKO, G. I. (1993), Phenotypic Characterization of CD7 +, CD3 +, and CD8+ Lymphocytes From First Trimester Human Decidua Using Two-Color Flow Cytometry. American Journal of Reproductive Immunology, 29: 5–16. doi: 10.1111/j.1600-0897.1993.tb00831.x
- Issue online: 9 MAY 2013
- Version of Record online: 9 MAY 2013
- Accepted August 4, 1992
- Human decidua;
- early pregnancy;
- decidual lymphocytes;
- flow cytometry
PROBLEM: There is increasing evidence that decidual lymphocytes play a major role in local interactions at the fetomaternal interface.
METHOD: In this paper we use two-color flow cytometry to delineate the phenotype of lymphocytes obtained from human early decidua by mechanical dispersal technique.
RESULTS: The most abundant decidual lymphocytes expressed CD7, CD38, CD56, and CD2 markers, relatively small proportions of CD3 +, CD8 +, CD4 +, CD16 +, CD45RA+, CD11b+, and Leu8+ cells were also present. The vast majority of decidual CD7+ lymphocytes expressed CD38, CD2, and CD56 markers and were CD3 −, CD8 −, CD 16 −, and CD57 −. Decidual CD3+ lymphocytes were weakly staining for TCR α/β, lacked T-cell receptor (TCR) γ/δ molecules, and ∼40% of them expressed HLA-DR. All decidual CD8+ lymphocytes were CD2+ and the majority of them expressed CD38, CD56, and CD7 markers and were CD3−at the same time CD8 + CD7− lymphocytes were found in decidua. According to the expression of the CD45RA marker, decidual CD8 + lymphocytes could be divided into two subsets: CD8 + CD45RA + CD56 + and CD8 + CD45RA −.
CONCLUSIONS: These data clearly demonstrate that decidual lymphocytes display phenotypical features different from those of their counterparts in peripheral blood.