Separation of a 170-K Delayed-Implantation-Associated Protein With Inhibitory Activity on Trophoblast Outgrowth and RNA Synthesis by Mouse Embryo


Department of Obstetrics and Gynaecology, Grace Hospital, University of British Columbia, Vancouver, BC, V6H 3V5, Canada.


PROBLEM: To screen the uterine protein responsible for embryonic dormancy associated with delayed implantation.

METHOD: Uterine protein extracts and sera from mice in which delayed implantation had been induced and those from pregnant mice were separated by three steps of chromatography and SDS-PAGE by monitoring an inhibitory activity on trophoblast outgrowth. The presence of the separated protein in the uterine luminal fluid was assessed. Effect of the protein on cell proliferation and RNA synthesis by blastocysts were assessed.

RESULTS: A 170-K protein was found in the uterine tissue as well as uterine luminal fluid associated with delayed implantation. The 170-K protein suppressed RNA synthesis by approximately 50% and cell proliferation in blastocysts.

CONCLUSION: A 170-K protein is secreted into the uterine lumen during delayed implantation period. The ability of 170-K protein to suppress RNA synthesis and cell proliferation may play a role in regulation of embryonic dormancy associated with delayed implantation.