H5D12 Monoclonal Antibody Identifies a 24-kD Immunosuppressor Factor Produced by Human Chorio Carcinoma Cells
Version of Record online: 9 MAY 2013
American Journal of Reproductive Immunology
Volume 32, Issue 2, pages 101–107, September 1994
How to Cite
Bose, R. and Berczi, A. (1994), H5D12 Monoclonal Antibody Identifies a 24-kD Immunosuppressor Factor Produced by Human Chorio Carcinoma Cells. American Journal of Reproductive Immunology, 32: 101–107. doi: 10.1111/j.1600-0897.1994.tb01100.x
- Issue online: 9 MAY 2013
- Version of Record online: 9 MAY 2013
- Accepted October 11, 1993
- monoclonal antibody;
- immunosuppressor factor;
- chorio carcinoma
PROBLEM: The purpose of this study was to identify and characterize embryo-associated immunosuppressor factor (EASF) secreted by chorio carcinoma cells with the help of EASF-specific monoclonal antibody H5D12 (raised against EASF purified from embryo growth media of in vitro fertilized human ova).
METHOD: Paraffin-embedded slides of human chorio carcinoma as well as control cell lines were prepared, and immunohistochemistry was done by the avidin-biotin-peroxidase technique. EASF was affinity purified using H5D12-Sepharose 4B from culture media of cell lines and analyzed for immunosuppressive activity (by Concanavalin-A-induced lymphocyte proliferation assay) and molecular weight identity (by metabolic-labelling studies with 35S-methionine followed by immunoprecipitation and SDS-PAGE).
RESULTS: H5D12 showed intense immunostaining of BeWo chorio carcinoma cells. Bio-synthetic labeling studies identifies this factor as a 24-kD molecule, and EASF bioassay indicates that this factor possesses immunosuppressive activity. No such immunosuppressive activity or similar molecules were identified when control cell lines were analyzed.
CONCLUSIONS: Monoclonal antibody H5D12 recognizes a 24-kD factor with immunosuppressive activity that is secreted by chorio carcinoma cells, which suggests that this is a unique factor and may be one of the key regulators of reproductive functions.