Use of Lymphocyte Platelet Binding Assay for Detecting a Preimplantation Factor: A Quantitative Assay
Article first published online: 9 MAY 2013
American Journal of Reproductive Immunology
Volume 32, Issue 3, pages 133–138, October 1994
How to Cite
Barnea, E. R., Lahijani, K. I., Roussev, R., Barnea, J. D. and Coulam, C. B. (1994), Use of Lymphocyte Platelet Binding Assay for Detecting a Preimplantation Factor: A Quantitative Assay. American Journal of Reproductive Immunology, 32: 133–138. doi: 10.1111/j.1600-0897.1994.tb01103.x
- Issue published online: 9 MAY 2013
- Article first published online: 9 MAY 2013
- Accepted July 29, 1994
- preimplantation factor;
- lymphocyte/platelet binding assay;
- early pregnancy test
PROBLEM: To evaluate the ability of the lymphocyte/platelet binding assay to identify a preimplantation factor (PIF).
METHOD: Percentages of binding of lymphocytes by platelets in the presence of sera from 30 known pregnant and 30 nonpregnant individuals were compared using a novel lymphocyte/platelet binding assay. The assay is performed using a combination of a heat inactivated sera with donor O+ lymphocytes, activated complement and an antibody against CD2(T11, Ortho Pharmaceuticals).
RESULTS: In nonpregnant females (23.6 ± 6.5%) and males (17.7 ± 4.7%) the percentage of lymphocytes bound by platelets was significantly different from pregnant women (56.1 ± 15.9%) (P < 0.0001). Serial sampling of blood in five women undergoing IVF/ET who had normal pregnancies showed the detection of PIF by 4 days after transfer. The lymphocyte/platelet binding assay was not influenced by hCG, progesterone and estradiol. The interassay and intraassay variabilities were <3%.
CONCLUSIONS: The lymphocyte/platelet binding assay is a simple, reproducible, specific and cost efficient assay for measurement of PIF. Application of this assay will provide investigative and diagnostic tools for identifying and monitoring early pregnancy events.