I. Organ Culture of Amniochorionic Membrane In Vitro
Article first published online: 9 MAY 2013
American Journal of Reproductive Immunology
Volume 32, Issue 3, pages 184–187, October 1994
How to Cite
Fortunato, S. J., Menon, R., Swan, K. F. and Lyden, T. W. (1994), I. Organ Culture of Amniochorionic Membrane In Vitro. American Journal of Reproductive Immunology, 32: 184–187. doi: 10.1111/j.1600-0897.1994.tb01112.x
- Issue published online: 9 MAY 2013
- Article first published online: 9 MAY 2013
- Accepted May 31, 1994
- organ culture;
PROBLEM: The purpose of the study was to develop a novel method of amniochorionic membrane culture aimed at maintaining tissue integrity.
METHOD: Amniochorionic membranes were collected from women prior to labor, undergoing elective cesarean section, with no history of infection or pregnancy related complication. Fetal membranes were maintained in culture for up to ten days. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a “house keeping” gene and inflammatory cytokine mRNA was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) at various periods of culture. mRNA localization was performed by in situ hybridization.
RESULTS: GAPDH gene expression was seen throughout the culture period. Inflammatory cytokine mRNA (IL-1α, IL-1β and IL-6) were also detected during culture. Cellular and tissue morphology appeared normal.
CONCLUSIONS: The culture technique we propose is a simple organ explant system which maintains the morphology and autocrine/paracrine relationships within this tissue.