II. Expression of TNF-α and TNFR p55 in Cultured Amniochorion
Version of Record online: 9 MAY 2013
American Journal of Reproductive Immunology
Volume 32, Issue 3, pages 188–193, October 1994
How to Cite
Fortunato, S. J., Menon, R. and Swan, K. F. (1994), II. Expression of TNF-α and TNFR p55 in Cultured Amniochorion. American Journal of Reproductive Immunology, 32: 188–193. doi: 10.1111/j.1600-0897.1994.tb01113.x
- Issue online: 9 MAY 2013
- Version of Record online: 9 MAY 2013
- Accepted May 31, 1994
- preterm labor;
PROBLEM: Preterm labor and PROM are major complications of pregnancy. We have reported the possible role of amniochorionic membrane as it relates to the production of cytokines and the early onset of labor. Amniochorion is capable of responding to an infectious process with the production of IL-6 and IL-1β. Here we examine the expression of TNF-α and TNFR in amniochorion.
METHOD: Amniochorionic membranes were collected and maintained in an organ explant system. Samples were frozen and/or fixed for RT-PCR, in situ hybridization, and immunocytochemistry.
RESULTS: RT-PCR demonstrated mRNA for TNF-α and in situ hybridization localized mRNA in chorion and amnion. Immunocytochemistry demonstrated TNF-α peptide in amnion but not in chorion. Immunocytochemical localization of TNFR indicates presence of that peptide in both amnion and chorion.
CONCLUSIONS: We conclude that the fetal membranes are sources of TNF-α and TNFR, supporting our previous work indicating that fetal membranes are active participants in the response to intraamniotic infection.