Decay-Accelerating Factor Is Expressed in the Human Endometrium and May Serve as the Attachment Ligand for Dr Pili of Escherichia coli

Authors

  • Anil Kaul,

    1. Department of Obstetrics and Gynecology, The University of Texas Medical Branch at Galveston, Galveston, Texas
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  • Bogdan J. Nowicki MD, PhD,

    Corresponding author
    1. Department of Obstetrics and Gynecology, The University of Texas Medical Branch at Galveston, Galveston, Texas
    2. Department of Microbiology, The University of Texas Medical Branch at Galveston, Galveston, Texas
      Department of Obstetrics & Gynecology, School of Medicine, The University of Texas Medical Branch at Galveston, 301 University Boulevard, Galveston, TX 77555-0587.
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  • Mark G. Martens,

    1. Department of Obstetrics and Gynecology, The University of Texas Medical Branch at Galveston, Galveston, Texas
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  • Pawel Goluszko,

    1. Department of Obstetrics and Gynecology, The University of Texas Medical Branch at Galveston, Galveston, Texas
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  • Audrey Hart,

    1. Department of Obstetrics and Gynecology, The University of Texas Medical Branch at Galveston, Galveston, Texas
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  • Manubai Nagamani,

    1. Department of Obstetrics and Gynecology, The University of Texas Medical Branch at Galveston, Galveston, Texas
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  • Dhruv Kumar,

    1. Department of Pathology, The University of Texas Medical Branch at Galveston, Galveston, Texas
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  • Tuan Q. Pham,

    1. Department of Microbiology, The University of Texas Medical Branch at Galveston, Galveston, Texas
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  • Stella Nowicki

    1. Department of Obstetrics and Gynecology, The University of Texas Medical Branch at Galveston, Galveston, Texas
    2. Department of Microbiology, The University of Texas Medical Branch at Galveston, Galveston, Texas
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Department of Obstetrics & Gynecology, School of Medicine, The University of Texas Medical Branch at Galveston, 301 University Boulevard, Galveston, TX 77555-0587.

Abstract

PROBLEM: We evaluated the hypothesis that different tissue substructures in uteri may express decay accelerating factor (DAF), a complement regulatory protein that also may serve as ligand for bacterial attachment.

METHOD: Purified Dr pili, anti-Dr pili IgG, anti-DAF (SCR-3) IgG, and fluoresceinisothiocyanate-conjugated secondary IgG were used for binding and inhibition experiments. RESULT: We observed staining of endometrial glands, spiral arterioles, and myometrial arteries with Dr adhesin (pili) and anti-DAF (SCR-3) IgG, and found variation in distribution and amount of Dr ligands in different individuals. Anti-DAF (SCR-3) IgG blocked the binding of Dr pili to the endometrium.

CONCLUSION: Presence of DAF in endometrium may protect tissues from complement-induced damage. Differences between individuals in DAF density in the endometrium may affect sensitivity to attachment of Dr-bearing E. coli and/or complement activation.

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