Seminal Fluid and the Expression of MHC Class I Antigens in the Placenta of the Rat
Article first published online: 9 MAY 2013
American Journal of Reproductive Immunology
Volume 33, Issue 5, pages 367–372, May 1995
How to Cite
KANBOUR-SHAKIR, A., ARMSTRONG, D. T., ROULEAU, A., KUNZ, H. W. and GILL, T. J. (1995), Seminal Fluid and the Expression of MHC Class I Antigens in the Placenta of the Rat. American Journal of Reproductive Immunology, 33: 367–372. doi: 10.1111/j.1600-0897.1995.tb00905.x
- Issue published online: 9 MAY 2013
- Article first published online: 9 MAY 2013
- Accepted November 14, 1994
- Seminal fluid;
- placental MHC antigens;
- MHC antigen expression;
- embryo transfer;
PROBLEM: To determine whether seminal fluid influences the expression of MHC class I antigens on the surface of basal trophoblast cells in the placenta of the rat.
METHODS: Transfer of DA × DA embryos into a WF (allogeneic) or DA (syngeneic) recipient made pseudopregnant by hormonal treatment followed by mating with a vasectomized male (seminal fluid) or by mechanical stimulation (no seminal fluid). Antigen expression was determined by electron microscopic immunocytochemistry using the appropriate gold-labeled monoclonal antibodies.
RESULTS: Seminal fluid did not affect the expression of MHC class I antigens on the surface of the basal trophoblast in either allogeneic or syngeneic matings.
CONCLUSIONS: The suppression of the expression of paternal class I antigens on the surface of the basal trophoblast cells in allogeneic pregnancies most likely occurs at the genome level shortly after fertilization.