Evidence for a Leukocyte Adhesion Factor Produced by the Early Embryo
Version of Record online: 9 MAY 2013
American Journal of Reproductive Immunology
Volume 34, Issue 1, pages 20–25, July 1995
How to Cite
CHECK, J. H., BOLLENDORF, A. and ASKARI, H. A. (1995), Evidence for a Leukocyte Adhesion Factor Produced by the Early Embryo. American Journal of Reproductive Immunology, 34: 20–25. doi: 10.1111/j.1600-0897.1995.tb00915.x
- Issue online: 9 MAY 2013
- Version of Record online: 9 MAY 2013
- Accepted November 22, 1994
- Platelet rosette formation;
- leukocyte adhesion factor
PROBLEM: To determine if the embryo may induce adhesive molecules needed for implantation.
METHOD: Determination of whether platelet rosetting around lymphocytes might occur when exposed to sera from pregnant, but not nonpregnant patients and from culture fluid from embryos but not oocytes.
RESULTS: 90.2% of women with positive sera beta human chorionic gonadotropin (P-hCG) levels taken at least 12 days postovulation demonstrated platelet rosette factor (PRF) vs only 18.7% when β-hCG was negative. Using mid-luteal phase sera in women receiving hCG injection 1 wk before, 64.7% had positive PRF when serial β-hCG levels were positive as did 100% of samples taken from in vitro fertilization (IVF) patients; however, only 15.3% were positive with negative serial hCG levels. Culture media from fertilized oocytes and embryos tested positive for PRF, but follicular fluid and media from unfertilized oocytes were negative.
CONCLUSION: The early embryo secretes a factor(s) that gains access to maternal serum and promotes increased lymphocyte/platelet adhesiveness.