Interleukin-1β Induces Cyclooxygenase-2 in Cultured Human Decidual Cells
Version of Record online: 9 MAY 2013
American Journal of Reproductive Immunology
Volume 34, Issue 2, pages 65–71, August 1995
How to Cite
KENNARD, E. A., ZIMMERMAN, P. D., FRIEDMAN, C. I. and KNISS, D. A. (1995), Interleukin-1β Induces Cyclooxygenase-2 in Cultured Human Decidual Cells. American Journal of Reproductive Immunology, 34: 65–71. doi: 10.1111/j.1600-0897.1995.tb00920.x
- Issue online: 9 MAY 2013
- Version of Record online: 9 MAY 2013
- Accepted January 24, 1995
PROBLEM: The purpose of this study was to examine the hypothesis that interleukin-1β (IL-1β)-elicited increases in decidual prostaglandin E2and F2α (PGE2 and PGF2α) biosynthesis are due to the de novo expression of the inducible isoform of cyclooxygenase (i.e., COX-2).
METHOD: Primary human decidual cell cultures were established from term placentas delivered by cesarean section. After 8 days in vitro, when the cultures secreted immunoreactive prolactin, the cells were incubated for 24 h in serum-free medium, and then challenged with IL-1β from 1 to 48 h. PGE2 and PGF2α content in the media were measured by specific radioimmunoassays.
RESULTS: IL-1β stimulated a time-dependent enhancement in PGE2 and PGF2α production, with PGF2α synthesis predominating over PGE2. IL-1β also induced a dose-dependent increase in the output of both arachidonic acid metabolites. When Northern blots of IL-1β-treated and control cells were probed with cDNAs encoding either COX-1 or COX-2 isoforms or an oligonucleotide probe encoding a portion of the human β-actin, we detected a time-and dose-dependent increase in the steady-state levels of COX-2, but not COX-1 or β-actin mRNA transcripts. Moreover, the expression of COX-2 mRNA in IL-1β-stimulated cells was superinduced by preincubation with cycloheximide, but completely abolished by actinomycin D.
CONCLUSIONS: Taken together, the data suggest that COX-2 mRNA expression is largely responsible for the robust increase in PG formation seen in IL-1β-treated decidual cells.