Can the Immunobead Assay for Detecting Sperm Antibodies in Fresh Samples Be Reproduced in Cryopreserved/Thawed Human Spermatozoa?
Article first published online: 9 MAY 2013
American Journal of Reproductive Immunology
Volume 34, Issue 2, pages 116–119, August 1995
How to Cite
MELO-ABREU, S., BATTISH, S., BROWN, K., TURECK, R., HEYNER, S. and BLASCO, L. (1995), Can the Immunobead Assay for Detecting Sperm Antibodies in Fresh Samples Be Reproduced in Cryopreserved/Thawed Human Spermatozoa?. American Journal of Reproductive Immunology, 34: 116–119. doi: 10.1111/j.1600-0897.1995.tb00926.x
- Issue published online: 9 MAY 2013
- Article first published online: 9 MAY 2013
- Accepted February 6, 1995
- Immunobead assay;
PROBLEM: To evaluate the reproducibility of the Immunobead Assay (IBA) on sperm samples before and after cryopreservation.
METHOD: Sperm samples (fresh and post-thaw) from known antibody negative donors (N=20) were evaluated for percent immunobead binding by IBA following incubation with known antibody-positive serum.
RESULTS: In both fresh and thawed negative samples, the mean sperm head binding was 0.5% ± 0.5, the mean sperm tail binding was 2.0% ± 2.0 and the mean sperm head-tail binding was 3.0% ± 2.0 for IgG, IgA and IgM type antibodies, respectively. The same samples exposed to positive sera showed 40.0% ± 10.0 mean head binding, 7.0% ± 8.0 mean tail binding and 47.0% ± 11.0 mean head-tail binding.
CONCLUSIONS: IBA is highly reproducible for detecting sperm antibodies in both fresh and cryopreserved/thawed samples of human spermatozoa.