• Immunobead assay;
  • sperm;
  • cryopreservation

PROBLEM: To evaluate the reproducibility of the Immunobead Assay (IBA) on sperm samples before and after cryopreservation.

METHOD: Sperm samples (fresh and post-thaw) from known antibody negative donors (N=20) were evaluated for percent immunobead binding by IBA following incubation with known antibody-positive serum.

RESULTS: In both fresh and thawed negative samples, the mean sperm head binding was 0.5% ± 0.5, the mean sperm tail binding was 2.0% ± 2.0 and the mean sperm head-tail binding was 3.0% ± 2.0 for IgG, IgA and IgM type antibodies, respectively. The same samples exposed to positive sera showed 40.0% ± 10.0 mean head binding, 7.0% ± 8.0 mean tail binding and 47.0% ± 11.0 mean head-tail binding.

CONCLUSIONS: IBA is highly reproducible for detecting sperm antibodies in both fresh and cryopreserved/thawed samples of human spermatozoa.