Hematopoietic Stem Cell Antigen-1 (Sca-1) Expression in Different Lymphoid Tissues of Female Mice Treated With GnRH Agonist
Article first published online: 9 MAY 2013
American Journal of Reproductive Immunology
Volume 34, Issue 4, pages 257–266, October 1995
How to Cite
RAO, L. V., CLEVELAND, R.P., KIMMEL, R.J. and ATAYA, K.M. (1995), Hematopoietic Stem Cell Antigen-1 (Sca-1) Expression in Different Lymphoid Tissues of Female Mice Treated With GnRH Agonist. American Journal of Reproductive Immunology, 34: 257–266. doi: 10.1111/j.1600-0897.1995.tb00950.x
- Issue published online: 9 MAY 2013
- Article first published online: 9 MAY 2013
- Accepted May 16, 1995
- Lupron depot;
- immune system;
- bone marrow;
- lymphoid tissues
PROBLEM: Our earlier studies have demonstrated a general suppression of leukocyte maturation upon GnRH agonist treatment in mice and suggested a potential effect at an early stem cell stage of leukocyte development.
METHOD: Three-week old Balb/c and C57BL/6 female mice received 50 μlg injections of Lupron depot or placebo. Sequential changes in Sca-1+ cells in the bone marrow, thymus, blood and spleen were studied by flow cytometry.
RESULTS: In bone marrow, the absolute numbers of Sca-1+ cells were significantly decreased at 2 weeks in C57BL/6 mice whereas a decreasing trend was noted in Balb/c mice following agonist administration. Concomitantly, thymocytes expressing Sca-1+ cells were significantly increased at 2 weeks in C57BL/6 mice, but were significantly decreased in Balb/c mice. Significant decreases in Sca-1+ cells were also observed in spleen and blood in Balb/c mice whereas no significant differences were observed in C57BL/6 mice.
CONCLUSIONS: These data suggest GnRH agonists affect hematopoietic stem cell development in mice. The effects observed vary with different genetic backgrounds. In Balb/c mice these effects are more pronounced, and appear to result in the inhibition of stem cell maturation. In contrast, GnRH agonist enhances stem cell maturation in C57BL/6 mice.