Metalloproteinase Secretion by Endometrial Leukocyte Subsets
Article first published online: 9 MAY 2013
American Journal of Reproductive Immunology
Volume 34, Issue 5, pages 299–310, November 1995
How to Cite
SHI, W., MOGNETTI, B., CAMPANA, A. and BISCHOF, P. (1995), Metalloproteinase Secretion by Endometrial Leukocyte Subsets. American Journal of Reproductive Immunology, 34: 299–310. doi: 10.1111/j.1600-0897.1995.tb00956.x
- Issue published online: 9 MAY 2013
- Article first published online: 9 MAY 2013
- Accepted July 1, 1994
- Human endometrium/decidua;
- collagenase assay;
- leukocyte subset;
- large granular lymphocyte
PROBLEM: Human endometrium contains up to 40% leukocytes. Since their function is unclear, we cultured these cells separately to analyze their proteolytic potential in vitro.
METHOD: Endometrium was obtained from hysterectomies or from first trimester abortion. After collagenase digestion, the different leukocyte subsets (CD56, CD3, CD19, CD16, CD45) were immuno-separated and cultured. Gelatine zymography and a quantitative type IV gelatinase assay were run on all culture supernatants.
RESULTS: On zymography, all subtypes of endometrial leukocytes released gelatinases. The 72-kDa gelatinase was absent from peripheral leukocyte supernatants, whereas it was secreted by all endometrial leukocytes. The 92-kDa gelatinase was present in all culture supernatants. Large granular lymphocytes (CD56+, LGL) expressed the highest type VI gelatinase activity as compared to all other leukocytes. This activity increased during the cycle in LGL and T cells but remained unchanged in glandular and stromal cells.
CONCLUSIONS: Endometrial leukocytes, particularly LGL, secrete significant amounts of gelatinases.