Regulation of Classical HLA Class I Genes in Human Choriocarcinoma Cells by Nuclear Proteins Binding to MHC Class I Regulatory Elements
Article first published online: 9 MAY 2013
American Journal of Reproductive Immunology
Volume 34, Issue 5, pages 323–331, November 1995
How to Cite
WAKIMOTO, A., OHASHI, K., KOYAMA, M., KATO, M., TSUTSUI, T., SAJI, F. and TANIZAWA, O. (1995), Regulation of Classical HLA Class I Genes in Human Choriocarcinoma Cells by Nuclear Proteins Binding to MHC Class I Regulatory Elements. American Journal of Reproductive Immunology, 34: 323–331. doi: 10.1111/j.1600-0897.1995.tb00959.x
- Issue published online: 9 MAY 2013
- Article first published online: 9 MAY 2013
- Accepted December 6, 1994
- Classical HLA class I;
- human choriocarcinoma cells;
- nuclear protein;
- MHC class I regulatory element (CRE)
PROBLEM: The regulation of classical HLA class I genes in choriocarcinoma have been reported.
METHODS: We determined whether four choriocarcinoma cell lines expressed classical HLA class I or HLA-G by a reverse transcription-polymerase chain reaction (RT-PCR) and studied the regulatory mechanism of classical class I using a gel mobility shift assay.
RESULTS: NUC1 and SCH expressed classical class I but not HLA-G. GCH1 and Jar did neither. Nuclear protein binding to the class I regulatory element (CRE) was detected in NUC1 and SCH. Interferon-γ augmented both classical class I expression and the DNA-protein complex in NUC1. The DNA-protein complex was not observed in GCH1, and Jar showed a CRE-binding protein with different electrophoretic mobility and binding affinity from that of SCH and NUC1.
CONCLUSION: The CRE is one of the regulatory elements of classical HLA class I genes in choriocarcinoma cells.