Expression of Phosphatidylserine-Dependent Antigens on the Surface of Differentiating BeWo Human Choriocarcinoma Cells
Article first published online: 9 MAY 2013
American Journal of Reproductive Immunology
Volume 33, Issue 1, pages 114–121, January 1995
How to Cite
Rote, N. S., Chang, J., Katsuragawa, H., Ng, A. K., Lyden, T. W. and Mori, T. (1995), Expression of Phosphatidylserine-Dependent Antigens on the Surface of Differentiating BeWo Human Choriocarcinoma Cells. American Journal of Reproductive Immunology, 33: 114–121. doi: 10.1111/j.1600-0897.1995.tb01147.x
- Issue published online: 9 MAY 2013
- Article first published online: 9 MAY 2013
- Accepted August 25, 1994
- Antiphospholipid antibody;
PROBLEM: Antiphospholipid antibodies (aPLs) are associated with pregnancy loss, pregnancy-induced hypertension, and intrauterine growth retardation. We have previously reported that phosphatidylserine (PS)-dependent antigens are expressed in formalin-fixed cells concurrent with differentiation in a choriocarcinoma model (BeWo) of cytotrophoblast. That study, however, could not differentiate between cytoplasmic or surface antigen expression.
METHOD: Three monoclonal aPLs that differentiate between PS- and cardiolipin (CL)-dependent antigens were reacted with BeWo, with or without forskolin activation, before fixation, and antibody binding was evaluated by immunoperoxidase techniques.
RESULTS: Activation with forskolin induced a PS-dependent antigenic determinant on the surface on BeWo cells. CL-reactive monoclonal antibodies did not react with the cell surface, whether forskolin treated or not.
CONCLUSION: These observations demonstrate that a PS-dependent antigen is expressed on the surface of a model of differentiating cytotrophoblastic cells and should be accessible in vivo to circulating aPLs.