Globulin-Enriched Protein Supplements Shorten the Pre-Compaction Mitotic Interval and Promote Hatching of Murine Embryos

Authors

  • Dr. E. Gayle Schneider,

    Corresponding author
    1. Division of Reproductive Endocrinology, Department of Obstetrics and Gynecology, East Carolina University School of Medicine, Greenville, North Carolina
      ECU Women's Physicians, 2305 Executive Park West, Greenville, NC 27834.
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  • C. Calvin Hayslip

    1. Division of Reproductive Endocrinology, Department of Obstetrics and Gynecology, East Carolina University School of Medicine, Greenville, North Carolina
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ECU Women's Physicians, 2305 Executive Park West, Greenville, NC 27834.

Abstract

PROBLEM: To determine whether Synthetic Serum Substitute (SSS), which contains human globulins in addition to Human Serum Albumin (HSA), is superior to HSA alone as a protein supplement for embryo culture.

METHOD: Development of mouse zygotes to eight-cell/compacting morulae and to hatching/hatched blastocysts was assessed in Human Tubal Fluid (HTF) medium containing either SSS or HSA.

RESULTS: Although there was no difference in the overall blastocyst rate at 120 h in HTF+SSS versus HTF+HSA, significantly more embryos at 54 h were at the eight-cell/compacting morula stage in HTF+SSS. At 120 h, there were more hatching/hatched blastocysts in HTF+SSS, and hatching correlated with SSS concentration. Addition of isolated globulins to HSA significantly stimulated the number of hatching/hatched blastocysts. Hatching could be “rescued” by transfer of embryos grown in HTF+HSA to globulin-containing media and prevented by removal of globulins as late as the compacted morula stage (54 h).

CONCLUSIONS: SSS is superior to HSA alone for embryo culture. The stimulatory effects on mitosis and hatching may be mediated directly by globulins or by other components in the globulin-enriched fraction.

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