Anti-Seminal Plasma Antibodies Associated With Infertility: II. Comparative Investigation of Human Antibody Binding to Normo-, Astheno-, and Azoospermic Seminal Plasma Antigens
Article first published online: 6 SEP 2011
American Journal of Reproductive Immunology
Volume 36, Issue 4, pages 204–210, October 1996
How to Cite
Fichorova, R., Boulanov, I. and Stanislavov, R. (1996), Anti-Seminal Plasma Antibodies Associated With Infertility: II. Comparative Investigation of Human Antibody Binding to Normo-, Astheno-, and Azoospermic Seminal Plasma Antigens. American Journal of Reproductive Immunology, 36: 204–210. doi: 10.1111/j.1600-0897.1996.tb00164.x
- Issue published online: 6 SEP 2011
- Article first published online: 6 SEP 2011
- Accepted August 10, 1995
- Enzyme-linked immunosorbent assay;
- human seminal plasma antigens;
- immunologic infertility
PROBLEM: Sera from infertile patients with elevated reactivity against normozoospermic seminal plasma (NSP) have been selected to investigate human antibody binding to seminal fluid antigens present in abnormal ejaculates.
METHOD: Sera from 32 idiopathically infertile patients and 44 control sera from fertile individuals were examined by ELISA against: 1) pooled seminal plasma from asthenozoospermic ejaculates (AsthSP), 2) pooled seminal plasma from patients with aspermatogenic azoospermia (AzooSP), and 3) chromatographic fractions from NSP, AsthSP and AzooSP.
RESULTS: Of 32 patients positive for anti-NSP antibodies, only four exhibited increased reactivity to whole AsthSP and/or AzooSP, while 14 recognized antigens of different Mr and various distributions in the corresponding chromatographic fractions.
CONCLUSION: Targets of human anti-NSP antibodies might be lacking, less concentrated, and/or modified in AsthSP and AzooSP. These findings suggest their physiological importance and the possible relevance of the observed auto- and iso-immune responses to infertility.