Transforming Growth Factor-β2-Related-Decidual Suppressor Factor Is Not Related to TJ6 Protein

Authors

  • Fatima S. Merali,

    1. Departments of Medicine, Pathology, Obstetrics and Gynecology, Molecular Immunology, Virology and Inflamation Program, McMaster University, Hamilton, Ontario, Canada
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  • Petra C. Arck,

    1. Departments of Medicine, Pathology, Obstetrics and Gynecology, Molecular Immunology, Virology and Inflamation Program, McMaster University, Hamilton, Ontario, Canada
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  • Kenneth Beaman,

    1. Department of Microbiology and Immunology, The University of Health Sciences, The Chicago Medical School, Chicago, Illinois
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  • David A. Clark

    Corresponding author
    1. Departments of Medicine, Pathology, Obstetrics and Gynecology, Molecular Immunology, Virology and Inflamation Program, McMaster University, Hamilton, Ontario, Canada
      Room 3V39, 1200 Main Street West, Hamilton, Ontario, Canada L8N-3Z5.
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Room 3V39, 1200 Main Street West, Hamilton, Ontario, Canada L8N-3Z5.

Abstract

Transforming growth factor (TGF)-β2-related-decidual suppressor factor (DSF) and TJ6 protein are both immunosuppressive molecules present in murine and human pregnancy. Treatment of mice with either anti-TJ6 or anti-TGF-β2 neutralizing antibodies results in increased fetal loss. Western blots of supernatants from pregnant mouse decidua probed with anti-TJ6 (soluble form) showed a doublet at a similar molecular size as when the blot was probed with anti-TGF-β2 antibody. The problem is to determine whether TJ6 and DSF are the same protein. In order to determine if TJ6 and DSF are the same or different proteins, we used affinity column purified TGF-β2-DSF and stained Western blots with anti-TJ6. The TGF-β2-monoclonal antibody affinity column-purified DSF that stained with anti TGF-β2 was not reactive with anti-TJ6 antibody. TJ6 has only a 30% gene sequence homology and a 13% amino acid homology to TGF-β2. TJ6 and TGF-β2-related DSF appear to be different immunosuppressive proteins in decidua.

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